Journal: Clinical Epigenetics

Article Title: MEST mediates the impact of prenatal bisphenol A exposure on long-term body weight development

doi: 10.1186/s13148-018-0478-z

Figure Lengend Snippet: In vitro adipocyte differentiation from human MSCs: exposure to BPA (10 μM, 50 μM) compared to solvent control (EtOH 0.05%). a Real-time monitoring of cell differentiation (xCELLigence: normalized cell index) over a 17-day period (mean ± SD, n = 4). b Quantification of Oil Red O stained area (mean ± SD, n ≥ 20 from one experiment). c Exemplary histological Oil Red O staining of adipocytes (black bar = 100 μm). d qPCR data of genes involved in adipogenesis ( n ≥ 3) normalized to EtOH control ( Lep = leptin, LPL = lipoprotein lipase, PPAR γ = peroxisome proliferator activated receptor gamma, IRS2 = insulin receptor substrate 2, FASN = fatty acid synthase, SREBF 1 = sterol receptor element binding factor 1, ESR1 = estrogen receptor alpha). e Targeted MassARRAY analysis of MEST promoter methylation, shown are the measurement of the single CpG cg17580798 covered by the amplicon (gray bars, n = 3) and the mean of the MassARRAY amplicon (black bars). f qPCR data of MEST ( n ≥ 3, relative to EtOH control); * p < 0.05, ** p < 0.01, *** p < 0.001 from Student’s t test/ANOVA

Article Snippet: For adipocyte differentiation MSCs at passage 1–3 were seeded at 9600 cells/cm 2 and were cultured with Adipocyte Differentiation Initiation Medium (ADIM; ATCC Adipocyte Differentiation Toolkit PCS-500-050) for 4 days.

Techniques: In Vitro, Solvent, Control, Cell Differentiation, Staining, Binding Assay, Methylation, Amplification

Journal: Lab on a Chip

Article Title: Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes

doi: 10.1039/d2lc00245k

Figure Lengend Snippet: Parallel formation and cultivation of human adipose tissue-derived adult stem cells in 3D on-chip. A) Timeline of hASC self-aggregation in the cell culture chamber. Scale bar: 250 μm. B) Intrachip variability of the equivalent diameter of the 3D cell cultures. n = 6 biological repeats. C) Interchip variability of the equivalent diameter of formed hASC 3D cell cultures. n = 96 technical replicates per chip, all comparisons exhibited a p < 0.05 (one-way ANOVA) except for n.s. = not significant. D) Equivalent diameter of the hASC 3D cell cultures during on-chip culture. n = 4–12 technical replicates. E) Rates of successful retrieval of the hASC 3D cell cultures from the cell culture chambers. n = 3 biological replicates, * p < 0.05 (one-way ANOVA).

Article Snippet: Human adipose tissue-derived stem cells (hASCs; ASC-F-ZB) were purchased from ZenBio (NC, USA) and maintained in routine 2D cell culture using subcutaneous preadipocyte growth medium (PM-1; ZenBio) according to the supplier's protocol. hASCs at passages 6–8 were used for the experiments.

Techniques: Derivative Assay, Cell Culture